Identification of ε-Poly-L-lysine as an Antimicrobial Product from an Epichloë Endophyte and Isolation of Fungal ε-PL Synthetase Gene.
Identifieur interne : 000158 ( Main/Exploration ); précédent : 000157; suivant : 000159Identification of ε-Poly-L-lysine as an Antimicrobial Product from an Epichloë Endophyte and Isolation of Fungal ε-PL Synthetase Gene.
Auteurs : Enkhee Purev [Japon] ; Tatsuhiko Kondo [Japon] ; Daigo Takemoto [Japon] ; Jennifer T. Niones [Japon, Philippines] ; Makoto Ojika [Japon]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2020.
Abstract
The endophytic fungus Epichloë festucae is known to produce bioactive metabolites, which consequently protect the host plants from biotic and abiotic stresses. We previously found that the overexpression of vibA (a gene for transcription factor) in E. festucae strain E437 resulted in the secretion of an unknown fungicide. In the present study, the active substance was purified and chemically identified as ε-poly-L-lysine (ε-PL), which consisted of 28-34 lysine units. The productivity was 3.7-fold compared with that of the wild type strain E437. The isolated ε-PL showed inhibitory activity against the spore germination of the plant pathogens Drechslera erythrospila, Botrytis cinerea, and Phytophthora infestans at 1-10 μg/mL. We also isolated the fungal gene "epls" encoding ε-PL synthetase Epls. Overexpression of epls in the wild type strain E437 resulted in the enhanced production of ε-PL by 6.7-fold. Interestingly, overexpression of epls in the different strain E. festucae Fl1 resulted in the production of shorter ε-PL with 8-20 lysine, which exhibited a comparable antifungal activity to the longer one. The results demonstrate the first example of ε-PL synthetase gene from the eukaryotic genomes and suggest the potential of enhanced expression of vibA or/and epls genes in the Epichloë endophyte for constructing pest-tolerant plants.
DOI: 10.3390/molecules25051032
PubMed: 32106587
PubMed Central: PMC7179176
Affiliations:
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Niones</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Production Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Plant Production Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601</wicri:regionArea><wicri:noRegion>Nagoya 464-8601</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Philippine Rice Research Institute, Science City of Munoz, Nueva Ecija 3119, Philippines.</nlm:affiliation><country xml:lang="fr">Philippines</country><wicri:regionArea>Philippine Rice Research Institute, Science City of Munoz, Nueva Ecija 3119</wicri:regionArea><wicri:noRegion>Nueva Ecija 3119</wicri:noRegion></affiliation></author><author><name sortKey="Ojika, Makoto" sort="Ojika, Makoto" uniqKey="Ojika M" first="Makoto" last="Ojika">Makoto Ojika</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601</wicri:regionArea><wicri:noRegion>Nagoya 464-8601</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The endophytic fungus <i>Epichloë festucae</i> is known to produce bioactive metabolites, which consequently protect the host plants from biotic and abiotic stresses. We previously found that the overexpression of <i>vibA</i> (a gene for transcription factor) in <i>E. festucae</i> strain E437 resulted in the secretion of an unknown fungicide. In the present study, the active substance was purified and chemically identified as ε-poly-L-lysine (ε-PL), which consisted of 28-34 lysine units. The productivity was 3.7-fold compared with that of the wild type strain E437. The isolated ε-PL showed inhibitory activity against the spore germination of the plant pathogens <i>Drechslera erythrospila</i>, <i>Botrytis cinerea</i>, and <i>Phytophthora infestans</i> at 1-10 μg/mL. We also isolated the fungal gene "<i>epls</i>" encoding ε-PL synthetase Epls. Overexpression of <i>epls</i> in the wild type strain E437 resulted in the enhanced production of ε-PL by 6.7-fold. Interestingly, overexpression of <i>epls</i> in the different strain <i>E. festucae</i> Fl1 resulted in the production of shorter ε-PL with 8-20 lysine, which exhibited a comparable antifungal activity to the longer one. The results demonstrate the first example of ε-PL synthetase gene from the eukaryotic genomes and suggest the potential of enhanced expression of <i>vibA</i> or/and <i>epls</i> genes in the <i>Epichloë</i> endophyte for constructing pest-tolerant plants.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32106587</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>29</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>Feb</Month><Day>25</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Identification of ε-Poly-L-lysine as an Antimicrobial Product from an <i>Epichloë</i> Endophyte and Isolation of Fungal ε-PL Synthetase Gene.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1032</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules25051032</ELocationID><Abstract><AbstractText>The endophytic fungus <i>Epichloë festucae</i> is known to produce bioactive metabolites, which consequently protect the host plants from biotic and abiotic stresses. We previously found that the overexpression of <i>vibA</i> (a gene for transcription factor) in <i>E. festucae</i> strain E437 resulted in the secretion of an unknown fungicide. In the present study, the active substance was purified and chemically identified as ε-poly-L-lysine (ε-PL), which consisted of 28-34 lysine units. The productivity was 3.7-fold compared with that of the wild type strain E437. The isolated ε-PL showed inhibitory activity against the spore germination of the plant pathogens <i>Drechslera erythrospila</i>, <i>Botrytis cinerea</i>, and <i>Phytophthora infestans</i> at 1-10 μg/mL. We also isolated the fungal gene "<i>epls</i>" encoding ε-PL synthetase Epls. Overexpression of <i>epls</i> in the wild type strain E437 resulted in the enhanced production of ε-PL by 6.7-fold. Interestingly, overexpression of <i>epls</i> in the different strain <i>E. festucae</i> Fl1 resulted in the production of shorter ε-PL with 8-20 lysine, which exhibited a comparable antifungal activity to the longer one. The results demonstrate the first example of ε-PL synthetase gene from the eukaryotic genomes and suggest the potential of enhanced expression of <i>vibA</i> or/and <i>epls</i> genes in the <i>Epichloë</i> endophyte for constructing pest-tolerant plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Purev</LastName><ForeName>Enkhee</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kondo</LastName><ForeName>Tatsuhiko</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takemoto</LastName><ForeName>Daigo</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Production Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niones</LastName><ForeName>Jennifer T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>Department of Plant Production Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Philippine Rice Research Institute, Science City of Munoz, Nueva Ecija 3119, Philippines.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ojika</LastName><ForeName>Makoto</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-6671-8598</Identifier><AffiliationInfo><Affiliation>Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>18H04612</GrantID><Agency>Ministry of Education, Culture, Sports, Science and Technology</Agency><Country></Country></Grant><Grant><GrantID>16KT0145</GrantID><Agency>Japan Society for the Promotion of Science</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Epichloë</Keyword><Keyword MajorTopicYN="N">endophyte</Keyword><Keyword MajorTopicYN="N">fungicide</Keyword><Keyword MajorTopicYN="N">transcription factor</Keyword><Keyword MajorTopicYN="N">ε-polylysine</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Aug;80(16):4993-5000</Citation><ArticleIdList><ArticleId IdType="pubmed">24907331</ArticleId></ArticleIdList></Reference><Reference><Citation>J Basic Microbiol. 2012 Oct;52(5):513-22</Citation><ArticleIdList><ArticleId IdType="pubmed">22362499</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2002 Sep;162(1):89-101</Citation><ArticleIdList><ArticleId IdType="pubmed">12242225</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2004 Dec;41(12):1063-76</Citation><ArticleIdList><ArticleId IdType="pubmed">15531211</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biosci Bioeng. 2013 May;115(5):523-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23287500</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2013 Aug;16(4):480-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23850071</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2015 Jan;14(1):13-24</Citation><ArticleIdList><ArticleId IdType="pubmed">24906411</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2015 Oct 14;63(40):8787-92</Citation><ArticleIdList><ArticleId IdType="pubmed">26395226</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2019 Feb 6;67(5):1453-1462</Citation><ArticleIdList><ArticleId IdType="pubmed">30638374</ArticleId></ArticleIdList></Reference><Reference><Citation>Biopolymers. 1997 Sep;42(3):305-18</Citation><ArticleIdList><ArticleId IdType="pubmed">9279124</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechniques. 1997 Oct;23(4):686-90</Citation><ArticleIdList><ArticleId IdType="pubmed">9343693</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2006 Nov;73(2):321-31</Citation><ArticleIdList><ArticleId IdType="pubmed">16957897</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2005 Aug;57(4):1036-50</Citation><ArticleIdList><ArticleId IdType="pubmed">16091042</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2006 Aug;90(8):994-998</Citation><ArticleIdList><ArticleId IdType="pubmed">30781289</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioprocess Biosyst Eng. 2015 Sep;38(9):1705-13</Citation><ArticleIdList><ArticleId IdType="pubmed">25969386</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2017 Nov;22(11):939-948</Citation><ArticleIdList><ArticleId IdType="pubmed">28923242</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Genet Genomics. 2005 Aug;274(1):13-29</Citation><ArticleIdList><ArticleId IdType="pubmed">15991026</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2000 Oct;48(10):4687-92</Citation><ArticleIdList><ArticleId IdType="pubmed">11052720</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Biol. 2000 Mar;7(3):211-24</Citation><ArticleIdList><ArticleId IdType="pubmed">10712928</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2010 Sep;23(9):1130-42</Citation><ArticleIdList><ArticleId IdType="pubmed">20687803</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioprocess Biosyst Eng. 2017 Feb;40(2):271-283</Citation><ArticleIdList><ArticleId IdType="pubmed">27807681</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 1994 Jun;25(6):508-13</Citation><ArticleIdList><ArticleId IdType="pubmed">8082201</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Chem Biol. 2008 Dec;4(12):766-72</Citation><ArticleIdList><ArticleId IdType="pubmed">18997795</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosci Biotechnol Biochem. 2007 Jun;71(6):1470-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17587677</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2006 Dec;5(12):2161-73</Citation><ArticleIdList><ArticleId IdType="pubmed">17012538</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2019 Jul;20(7):961-975</Citation><ArticleIdList><ArticleId IdType="pubmed">31008572</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 1990 Nov;18(4):347-54</Citation><ArticleIdList><ArticleId IdType="pubmed">2147581</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2006 Mar;43(3):190-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16464624</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2002 Jul;68(7):3575-81</Citation><ArticleIdList><ArticleId IdType="pubmed">12089045</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2010 Aug;185(4):1271-82</Citation><ArticleIdList><ArticleId IdType="pubmed">20519745</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Oct;18(10):2807-21</Citation><ArticleIdList><ArticleId IdType="pubmed">17041146</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Mol Biol Rev. 2015 Sep;79(3):293-320</Citation><ArticleIdList><ArticleId IdType="pubmed">26136581</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li><li>Philippines</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Purev, Enkhee" sort="Purev, Enkhee" uniqKey="Purev E" first="Enkhee" last="Purev">Enkhee Purev</name></noRegion><name sortKey="Kondo, Tatsuhiko" sort="Kondo, Tatsuhiko" uniqKey="Kondo T" first="Tatsuhiko" last="Kondo">Tatsuhiko Kondo</name><name sortKey="Niones, Jennifer T" sort="Niones, Jennifer T" uniqKey="Niones J" first="Jennifer T" last="Niones">Jennifer T. Niones</name><name sortKey="Ojika, Makoto" sort="Ojika, Makoto" uniqKey="Ojika M" first="Makoto" last="Ojika">Makoto Ojika</name><name sortKey="Takemoto, Daigo" sort="Takemoto, Daigo" uniqKey="Takemoto D" first="Daigo" last="Takemoto">Daigo Takemoto</name></country><country name="Philippines"><noRegion><name sortKey="Niones, Jennifer T" sort="Niones, Jennifer T" uniqKey="Niones J" first="Jennifer T" last="Niones">Jennifer T. Niones</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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